Introduction:
Understanding the impact of skincare products on human skin cells isessential for developing safe and effective cosmetic formulations. In this study, weconducted an in vitro proliferation assay using the Human Keratinocytes (HaCaT)cell line to evaluate the effects of Suregro, a test product, on cell growth and viability.
Objective:
The objective of this study was to assess the potential cytotoxic effects of Suregro on Human Keratinocytes using the MTT assay, a widely used method for measuring cell viability.
Methods:
The Human Keratinocytes (HaCaT) cell line was cultured in a suitable growth medium. Suregro was prepared at various concentrations ranging from 1000 μg/mL to 7.8 μg/mL. The cells were exposed to these concentrations for a specific incubation period. Following the incubation, cell viability was determined using the MTT assay, which measures mitochondrial activity as an indicator of cell proliferation.
Results:
The data obtained from the proliferation assay revealed interesting findings. At the highest concentration of Suregro (1000 μg/mL), the percentage cell proliferation was measured to be 141.54±3.59%. As the concentration of Suregro decreased, the percentage of cell proliferation gradually decreased as well. These results suggest that Suregro has a dose-dependent effect on cell proliferation, potentially inhibiting cell growth at lower concentrations.
Discussion and Conclusion:
The findings of this study indicate that Suregro has the ability to induce cell proliferation in the Human Keratinocytes (HaCaT) cell line. However, further investigation is needed to determine the optimal concentration range for the desired effects and to evaluate the long-term impact of Suregro on cell viability and proliferation.
It is important to note that in vitro studies provide preliminary insights and serve as a starting point for further research. To fully assess the safety and efficacy of Suregro, additional studies such as in vivo experiments and clinical trials are necessary.
Archiving:
To ensure the integrity and availability of the data, Suregro will be stored for three months after the final report submission. The raw data, documents, and report generated from this study will be archived for a period of three years, enabling future reference and comparison with subsequent studies or regulatory requirements.
In conclusion, the in vitro proliferation assay conducted in this study sheds light on the effects of Suregro on Human Keratinocytes. By understanding the impact of skincare products on cellular behavior, we can develop safer and more effective formulations that promote healthy skin. Continued research and exploration will contribute to the advancement of cosmetic science and the development of innovative skincare solutions.